Retardation of immunofluorescence fading during microscopy.

Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy.

We have previously reported that the addition of para-phenylenediamine (PPD) to the glycerol used for mounting preparations stained for immunofluorescence with FITC conjugates results in a marked retardation of the fading that occurs during fluorescence microscopy. Studies of the kinetics of this photo-bleaching performed by microfluorimetry and on FITC conjugate in solution and the effects of ...

Rapid immunofluorescence microscopy for diagnosis of melioidosis.

An immunofluorescent (IF) method that detects Burkholderia pseudomallei in clinical specimens within 10 min was devised. The results of this rapid method and those of an existing IF method were prospectively compared with the culture results for 776 specimens from patients with suspected melioidosis. The sensitivities of both IF tests were 66%, and the specificities were 99.5 and 99.4%, respect...

Small quartz halogen lamps for immunofluorescence microscopy.

Reorganization of arrays of prekeratin filaments during mitosis. Immunofluorescence microscopy with multiclonal and monoclonal prekeratin antibodies.

Indirect immunofluorescent iabelling of different epithelial cell lines for intermediate filaments of the prekeratin type revealed prominent changes in the organization of prekeratin during mitosis. In three out of four cell lines tested (Henle-407, A-431 and HeLa cells) the filamentous prekeratin networks disappeared at the initiation of mitosis and the immunofluorescent labelling was concentr...

Study of direct immunofluorescence, immunofluorescence mapping and light microscopy in porphyria cutanea tarda.

BACKGROUND Even though porphyria cutanea tarda is the most frequent type of porphyria, there are few studies about its cutaneous physiopathology. OBJECTIVE To evaluate skin changes in porphyria cutanea tarda using light microscopy and direct immunofluorescence before and after treatment with chloroquine. To perform antigen immunomapping of bullae to study their level of cleavage. METHODS Li...